Evaluation of the anthelmintic properties of a traditional remedy based on a mixture of red algae using an in vitro assay on gastrointestinal nematodes of donkeys

The anthelmintic properties and composition of an Italian traditional remedy based on a red algae mixture (RAE) was assessed using the egg hatch test (EHT). ability different dilutions \((1.0, 5.0, 50, or 100%)\) RAE determined compared with positive negative controls against gastrointestinal nematode (GIN) donkeys. experiment performed in triplicate. Data were analysed ANOVA Tukey test. In mixture, Palisada tenerrima, Laurencia intricata spp. identified. \(100%\) able to totally inhibit hatch, showing efficacy comparable \((P < 0.05)\) that reference drug \((98.7%)\). An reduction \(89.5, 43.7\), \(23.4%\) observed at \(50, 5\) \(1%\) dilutions, respectively. conclusion, GIN donkeys concentration-dependent manner correlation coefficient \((R2)\) \(0.968\), corroborating its effect.